Hotspot

atpB-rbcL

LSC · 56534-57315 · 782 bp

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Pi 0.00621
MarkerSeek score 54.20
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
782
variable_sites
15
pi
0.00621
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 AATTGTGGAAATCCCGGGAC ACTCCGGGGTGTAGTAAGTC 50.0 55.0 58.2 58.2 889-912 bp 1.0 81.0
atpB-rbcL_p2 AATTGTGGAAATCCCGGGAC CTCCGGGGTGTAGTAAGTCA 50.0 55.0 58.2 58.2 888-911 bp 1.0 81.0
atpB-rbcL_p3 CAATTGTGGAAATCCCGGGA ACTCCGGGGTGTAGTAAGTC 50.0 55.0 58.2 58.2 890-913 bp 1.0 81.0
atpB-rbcL_p4 TCAATTGTGGAAATCCCGGG ACTCCGGGGTGTAGTAAGTC 50.0 55.0 58.2 58.2 891-914 bp 1.0 81.0
atpB-rbcL_p5 CAATTGTGGAAATCCCGGGA CTCCGGGGTGTAGTAAGTCA 50.0 55.0 58.2 58.2 889-912 bp 1.0 81.0