Hotspot

trnF-GAA-ndhJ

LSC · 50373-50964 · 592 bp

Pi 0.00273

MarkerSeek score 50.90

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 592
variable_sites: 4
pi: 0.00273
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	700-702 bp	1.0	79.4
trnF-GAA-ndhJ_p2	ATGCACAAGAAAGGGTCAGG	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	753-755 bp	1.0	79.0
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	690-692 bp	1.0	78.8
trnF-GAA-ndhJ_p4	ATGCACAAGAAAGGGTCAGG	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	743-745 bp	1.0	78.5
trnF-GAA-ndhJ_p5	GCACAAGAAAGGGTCAGGAT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	57.8	57.9	751-753 bp	1.0	78.1