Hotspot

psaA-ycf3

LSC · 44035-44641 · 607 bp

Pi 0.00141

MarkerSeek score 46.80

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 607
variable_sites: 3
pi: 0.00141
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GGGGCCCTTAGCTATTGTTC	TGGTTTGATCAAGCTGCTGA	55.0	45.0	58.0	57.7	837-839 bp	1.0	78.0
psaA-ycf3_p2	GTCTGGCCCATTCCTCAAAA	TGGTTTGATCAAGCTGCTGA	50.0	45.0	58.1	57.7	802-804 bp	1.0	77.6
psaA-ycf3_p3	GGGGCCCTTAGCTATTGTTC	GCTATAGCGCTTACTCCAGG	55.0	55.0	58.0	57.6	804-806 bp	1.0	77.4
psaA-ycf3_p4	AACTTCTGGTTCCGACGAAC	TGGTTTGATCAAGCTGCTGA	50.0	45.0	58.1	57.7	747-749 bp	1.0	77.1
psaA-ycf3_p5	GTCTGGCCCATTCCTCAAAA	GCTATAGCGCTTACTCCAGG	50.0	55.0	58.1	57.6	769-771 bp	1.0	77.0