Hotspot

trnC-GCA-rpoB

LSC · 20662-21872 · 1211 bp

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Pi 0.00448
MarkerSeek score 53.60
Primer available yes
Label trnC-GCA-rpoB

Feature Metadata

label_name
trnC-GCA-rpoB
feature_type
igs
region
LSC
length_bp
1211
variable_sites
17
pi
0.00448
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-rpoB_p1 AAAGGATTTGCAGTCCCCTG CTTCTCAAGTTCTTCCGCCA 50.0 50.0 58.1 57.8 1365-1505 bp 1.0 79.2
trnC-GCA-rpoB_p2 AAAGGATTTGCAGTCCCCTG TCTCAAGTTCTTCCGCCAAG 50.0 50.0 58.1 57.8 1363-1503 bp 1.0 79.2
trnC-GCA-rpoB_p3 AAAGGATTTGCAGTCCCCTG ACTTCTCAAGTTCTTCCGCC 50.0 50.0 58.1 57.8 1366-1506 bp 1.0 79.2
trnC-GCA-rpoB_p4 AAAGGATTTGCAGTCCCCTG TTCCGCCAAGCCTTGATTAA 50.0 45.0 58.1 57.8 1353-1493 bp 1.0 78.9
trnC-GCA-rpoB_p5 AAAGGATTTGCAGTCCCCTG CTTCCGCCAAGCCTTGATTA 50.0 50.0 58.1 58.2 1354-1494 bp 1.0 78.6