Hotspot

trnG-UCC-trnM-CAU

LSC · 14401-16359 · 1959 bp

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Pi 0.00156
MarkerSeek score 49.40
Primer available yes
Label trnG-UCC-trnM-CAU

Feature Metadata

label_name
trnG-UCC-trnM-CAU
feature_type
igs
region
LSC
length_bp
1959
variable_sites
7
pi
0.00156
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.96
markerseek_score
49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnM-CAU_p1 ACGAATCACACTTTTACCACT GCGAAAAGCCCTTTATCGGA 38.1 50.0 55.5 58.6 1982-2065 bp 1.0 50.3
trnG-UCC-trnM-CAU_p2 ACGAATCACACTTTTACCACT TCGAACCGATGACTTCTGTC 38.1 50.0 55.5 57.4 2028-2111 bp 1.0 50.2
trnG-UCC-trnM-CAU_p3 ACGAATCACACTTTTACCACT CGAACCGATGACTTCTGTCT 38.1 50.0 55.5 57.4 2027-2110 bp 1.0 50.1
trnG-UCC-trnM-CAU_p4 AGAACGAATCACACTTTTACCAC GCGAAAAGCCCTTTATCGGA 39.1 50.0 57.3 58.6 1985-2068 bp 1.0 49.2
trnG-UCC-trnM-CAU_p5 GAACGAATCACACTTTTACCACT GCGAAAAGCCCTTTATCGGA 39.1 50.0 57.3 58.6 1984-2067 bp 1.0 49.2