Hotspot

psbA-trnK-UUU

LSC · 1151-1378 · 228 bp

Pi 0.0113

MarkerSeek score 48.10

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 228
variable_sites: 9
pi: 0.0113
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	AAGACGGTTTTCGGTGCTAG	AAATGCAAGCACGATTTGGG	50.0	45.0	58.2	57.9	418-419 bp	1.0	79.6
psbA-trnK-UUU_p2	AAAGACGGTTTTCGGTGCTA	AAATGCAAGCACGATTTGGG	45.0	45.0	57.8	57.9	419-420 bp	1.0	79.3
psbA-trnK-UUU_p3	TAAAGACGGTTTTCGGTGCT	AAATGCAAGCACGATTTGGG	45.0	45.0	57.8	57.9	420-421 bp	1.0	79.3
psbA-trnK-UUU_p4	GCTTGTACTTTCGCGTCTCT	AAATGCAAGCACGATTTGGG	50.0	45.0	58.3	57.9	370-371 bp	1.0	79.1
psbA-trnK-UUU_p5	AACACCGAACCATCCAATGT	AAATGCAAGCACGATTTGGG	45.0	45.0	57.7	57.9	439-440 bp	1.0	78.9