Hotspot

ycf2

IRb · 95153-101980 · 6828 bp

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Pi 0.0274

MarkerSeek score 55.20

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRb
length_bp: 6828
variable_sites: 183
pi: 0.0274
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	ATTGAACAACCGGGAGCAAT	ATGCTCGTTCCAAGTTCGAA	45.0	45.0	58.1	57.8	1154-1199 bp	1.0	80.8
ycf2_p2	CCGCTTATGGGGTCAAATCA	ATGCTCGTTCCAAGTTCGAA	50.0	45.0	57.9	57.8	1507 bp	0.5	58.3
ycf2_p3	CCGCTTATGGGGTCAAATCA	AATCGGTCTTTTTCCGGCAT	50.0	45.0	57.9	58.2	829 bp	0.5	58.3
ycf2_p4	CCGCTTATGGGGTCAAATCA	CCCATGATCCTGAACCGATC	50.0	55.0	57.9	57.8	1355 bp	0.5	58.2
ycf2_p5	CCGCTTATGGGGTCAAATCA	GATCCCATGATCCTGAACCG	50.0	55.0	57.9	57.8	1358 bp	0.5	58.2