Hotspot

clpP

LSC · 77994-80076 · 2083 bp

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Pi 0.254

MarkerSeek score 65.10

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2083
variable_sites: 150
pi: 0.254
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.28
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CTAGTACATGTTCCTCGGCG	AAACGGCCGGGATCATTTAA	55.0	45.0	57.9	57.9	1362-2990 bp	1.0	90.0
clpP_p2	CTAGTACATGTTCCTCGGCG	ACCAAGGCAAACCCATGAAA	55.0	45.0	57.9	58.2	1322-2950 bp	1.0	89.4
clpP_p3	TGACATTTCGGATTGGCTGT	TGGACAGAAAGTAAACGGCC	45.0	50.0	57.8	58.1	2950 bp	0.5	57.5
clpP_p4	TGACATTTCGGATTGGCTGT	AAACGGCCGGGATCATTTAA	45.0	45.0	57.8	57.9	2938 bp	0.5	57.4
clpP_p5	TGCACCAACGGAAACCATAA	AGAATACGCAATGGTGGAGG	45.0	50.0	58.0	57.7	2940 bp	0.5	57.2