Hotspot

trnI-CAU-rpl23

IRa · 161890-162054 · 165 bp

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Pi 0.213
MarkerSeek score 74.70
Primer available yes
Label trnI-CAU-rpl23

Feature Metadata

label_name
trnI-CAU-rpl23
feature_type
igs
region
IRa
length_bp
165
variable_sites
35
pi
0.213
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-CAU-rpl23_p1 AGGTTCAATTCCTACTGGATGC TGCATATTTGATTCCATCCA 45.5 35.0 58.1 52.5 209-376 bp 1.0 48.3
trnI-CAU-rpl23_p2 GGTTCAATTCCTACTGGATGC TGCATATTTGATTCCATCCA 47.6 35.0 56.7 52.5 208-375 bp 1.0 48.3
trnI-CAU-rpl23_p3 GGTTCAATTCCTACTGGATGCA TGCATATTTGATTCCATCCA 45.5 35.0 58.4 52.5 208-375 bp 1.0 48.3
trnI-CAU-rpl23_p4 AGGTTCAATTCCTACTGGATGC TGCATATTTGATTCCATCCAT 45.5 33.3 58.1 53.3 209-376 bp 1.0 48.3
trnI-CAU-rpl23_p5 GGTTCAATTCCTACTGGATGC TGCATATTTGATTCCATCCAT 47.6 33.3 56.7 53.3 208-375 bp 1.0 48.3