Hotspot

ndhF-rpl32

SSC · 121372-122343 · 972 bp

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Pi 0.0763

MarkerSeek score 79.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 972
variable_sites: 70
pi: 0.0763
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAATAACAGGAAGGCGCACA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.1	58.0	2302-2337 bp	1.0	86.3
ndhF-rpl32_p2	AATAACAGGAAGGCGCACAA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.1	58.0	2301-2336 bp	1.0	86.3
ndhF-rpl32_p3	ATAACAGGAAGGCGCACAAA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.1	58.0	2300-2335 bp	1.0	86.3
ndhF-rpl32_p4	AATAACAGGAAGGCGCACAA	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.1	58.0	2302-2337 bp	1.0	86.3
ndhF-rpl32_p5	ATAACAGGAAGGCGCACAAA	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.1	58.0	2301-2336 bp	1.0	86.3