Hotspot

trnR-UCU-atpA

LSC · 12036-12456 · 421 bp

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Pi 0.105

MarkerSeek score 81.50

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 421
variable_sites: 43
pi: 0.105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	AGCAGAAGCCCTTTTGAAAGA	47.6	42.9	57.3	58.1	522-922 bp	1.0	64.3
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	GCAGAAGCCCTTTTGAAAGA	47.6	45.0	57.3	56.5	521-921 bp	1.0	60.9
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	AGCAGAAGCCCTTTTGAAAG	47.6	45.0	57.3	56.5	522-922 bp	1.0	60.8
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	GCAGAAGCCCTTTTGAAAGAA	47.6	42.9	57.3	57.3	521-921 bp	1.0	58.8
trnR-UCU-atpA_p5	GTTCAAATCCTATTGGACGCA	AGCAGAAGCCCTTTTGAAAGA	42.9	42.9	56.6	58.1	521-921 bp	1.0	58.6