Hotspot

rpl32-trnL-UAG

SSC · 116568-117211 · 644 bp

Back to Zanthoxylum

Pi 0.0212

MarkerSeek score 69.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 644
variable_sites: 89
pi: 0.0212
unique_haplotype_count: 29
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GCGTTGAAGGCTTTTTCGTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.2	58.0	756-786 bp	1.0	90.6
rpl32-trnL-UAG_p2	GCGTTGAAGGCTTTTTCGTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.2	58.0	757-787 bp	1.0	90.6
rpl32-trnL-UAG_p3	GCCAAAGATAAACGCGGAAG	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.8	58.0	1228-1269 bp	0.935484	87.1
rpl32-trnL-UAG_p4	GCCAAAGATAAACGCGGAAG	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.8	58.0	1229-1270 bp	0.935484	87.0
rpl32-trnL-UAG_p5	AGCCAAAGATAAACGCGGAA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.2	58.0	1229-1270 bp	0.935484	87.0