Hotspot

psaA-ycf3

LSC · 43971-44677 · 707 bp

Back to Zanthoxylum

Pi 0.014

MarkerSeek score 70.70

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 707
variable_sites: 46
pi: 0.014
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	AGCGCAGAATTGGTTGAAGA	50.0	45.0	57.9	58.1	813-857 bp	1.0	85.1
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	ATTGGTTGAAGATCACGGGG	50.0	50.0	57.9	57.9	805-849 bp	1.0	85.0
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	AGCTATTGCGCTTACTCCTG	50.0	50.0	57.9	57.8	846-890 bp	1.0	83.8
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	TCAAGCGCAGAATTGGTTGA	50.0	45.0	57.9	58.4	816-860 bp	1.0	82.7
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	CAAGCGCAGAATTGGTTGAA	50.0	45.0	57.9	57.6	815-859 bp	1.0	82.5