Hotspot

rpoB-trnC-GCA

LSC · 27999-29102 · 1104 bp

Back to Zanthoxylum

Pi 0.0167
MarkerSeek score 73.50
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1104
variable_sites
123
pi
0.0167
unique_haplotype_count
29
primer_available
yes
alignment_reliability
0.99
markerseek_score
73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 ATTCCCGCATTTCCATCTCC AAAAGGATTTGCAGTCCCCC 50.0 50.0 58.0 58.4 1174-1372 bp 1.0 88.5
rpoB-trnC-GCA_p2 ATTCCCGCATTTCCATCTCC ATCGTTTGTTGATCAGGCGA 50.0 45.0 58.0 57.9 1215-1413 bp 0.935484 87.8
rpoB-trnC-GCA_p3 ATTCCCGCATTTCCATCTCC GCGACACCCAGATTTGAACT 50.0 50.0 58.0 58.5 1199-1397 bp 1.0 87.4
rpoB-trnC-GCA_p4 ATTCCCGCATTTCCATCTCC GTTTGTTGATCAGGCGACAC 50.0 50.0 58.0 57.7 1212-1410 bp 0.935484 86.0
rpoB-trnC-GCA_p5 GCATTTCCATCTCCGAGCAT ATCGTTTGTTGATCAGGCGA 50.0 45.0 58.4 57.9 1209-1407 bp 0.935484 84.7