Hotspot

atpH-atpI

LSC · 14398-15673 · 1276 bp

Back to Zanthoxylum

Pi 0.0136

MarkerSeek score 66.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1276
variable_sites: 126
pi: 0.0136
unique_haplotype_count: 28
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TCTATGGAGGGACATCATTGA	45.0	42.9	58.0	55.5	1291-1357 bp	1.0	63.7
atpH-atpI_p2	AATAACGGAAGCGGCAGAAA	TCTATGGAGGGACATCATTGA	45.0	42.9	58.2	55.5	1292-1358 bp	1.0	62.5
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	ATCTATGGAGGGACATCATTGA	45.0	40.9	58.0	56.1	1292-1358 bp	1.0	60.2
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	ATCTATGGAGGGACATCATTGA	45.0	40.9	58.2	56.1	1293-1359 bp	1.0	59.0
atpH-atpI_p5	TAACGGAAGCGGCAGAAATC	TCTATGGAGGGACATCATTGA	50.0	42.9	58.6	55.5	1290-1356 bp	1.0	58.9