Hotspot

psbA-trnK-UUU

LSC · 1568-1837 · 270 bp

Back to Zanthoxylum

Pi 0.0156
MarkerSeek score 69.30
Primer available yes
Label psbA-trnK-UUU

Feature Metadata

label_name
psbA-trnK-UUU
feature_type
igs
region
LSC
length_bp
270
variable_sites
25
pi
0.0156
unique_haplotype_count
25
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-trnK-UUU_p1 CCGTTTTCGCGTCTCTCTAA ACTCCATCCGACTAGTTCCG 50.0 55.0 58.0 58.6 335-363 bp 1.0 85.2
psbA-trnK-UUU_p2 CCGTTTTCGCGTCTCTCTAA GAAAAATGCATGCACGGCTT 50.0 45.0 58.0 58.6 396-423 bp 1.0 85.0
psbA-trnK-UUU_p3 CCGTTTTCGCGTCTCTCTAA ATGAAAAATGCATGCACGGC 50.0 45.0 58.0 58.6 398-425 bp 1.0 84.4
psbA-trnK-UUU_p4 ATGTAAAGGCGGTTTTCGGT ACTCCATCCGACTAGTTCCG 45.0 55.0 58.1 58.6 392-420 bp 1.0 84.2
psbA-trnK-UUU_p5 ATGTAAAGGCGGTTTTCGGT GAAAAATGCATGCACGGCTT 45.0 45.0 58.1 58.6 453-480 bp 1.0 83.9