Hotspot

rps2

LSC · 16717-17499 · 783 bp

Back to Zantedeschia

Pi 0

MarkerSeek score 61.70

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 783
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	TGAAATCCAGGAATAGCCAA	CAACGGTCAATCCTCGGTAG	40.0	55.0	54.3	58.1	944-968 bp	1.0	51.7
rps2_p2	TTGAAATCCAGGAATAGCCA	CAACGGTCAATCCTCGGTAG	40.0	55.0	54.3	58.1	945-969 bp	1.0	51.7
rps2_p3	TGAAATCCAGGAATAGCCAA	AACGGTCAATCCTCGGTAGA	40.0	50.0	54.3	58.2	943-967 bp	1.0	51.0
rps2_p4	TGAAATCCAGGAATAGCCAA	ACGGTCAATCCTCGGTAGAA	40.0	50.0	54.3	58.2	942-966 bp	1.0	51.0
rps2_p5	TTGAAATCCAGGAATAGCCA	ACGGTCAATCCTCGGTAGAA	40.0	50.0	54.3	58.2	943-967 bp	1.0	51.0