Hotspot

rps18

LSC · 74414-74962 · 549 bp

Back to Zantedeschia

Pi 0

MarkerSeek score 59.00

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: LSC
length_bp: 549
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	CGTTCGGATCTTAAATGCATGA	AGTTTTGAGCCAATTGAAGAGT	40.9	36.4	57.2	56.5	538-634 bp	1.0	44.0
rps18_p2	TCGTTCGGATCTTAAATGCATG	AGTTTTGAGCCAATTGAAGAGT	40.9	36.4	57.2	56.5	539-635 bp	1.0	44.0
rps18_p3	TCGTTCGGATCTTAAATGCA	AGTTTTGAGCCAATTGAAGAGT	40.0	36.4	55.2	56.5	539-635 bp	1.0	44.0
rps18_p4	TTCGTTCGGATCTTAAATGCA	AGTTTTGAGCCAATTGAAGAGT	38.1	36.4	56.0	56.5	540-636 bp	1.0	44.0
rps18_p5	CGTTCGGATCTTAAATGCATGA	TGAAGTTTTGAGCCAATTGAAGA	40.9	34.8	57.2	57.1	541-637 bp	1.0	44.0