Hotspot

trnI-CAU-ycf2

IRb · 92543-92912 · 370 bp

Back to Zantedeschia

Pi 0.0415

MarkerSeek score 76.80

Primer available yes

Label trnI-CAU-ycf2

Feature Metadata

label_name: trnI-CAU-ycf2
feature_type: igs
region: IRb
length_bp: 370
variable_sites: 12
pi: 0.0415
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-ycf2_p1	GTTGGGCGCTTTAACCATTC	TGAACCGAATTTGATCCACGA	50.0	42.9	58.0	58.0	429-510 bp	1.0	77.6
trnI-CAU-ycf2_p2	GTTGGGCGCTTTAACCATTC	CTGAACCGAATTTGATCCACG	50.0	47.6	58.0	57.6	430-511 bp	1.0	74.8
trnI-CAU-ycf2_p3	AGTTGGGCGCTTTAACCATT	TGAACCGAATTTGATCCACGA	45.0	42.9	58.4	58.0	430-511 bp	1.0	74.7
trnI-CAU-ycf2_p4	TTGGGCGCTTTAACCATTCA	TGAACCGAATTTGATCCACGA	45.0	42.9	58.4	58.0	428-509 bp	1.0	74.6
trnI-CAU-ycf2_p5	GTTGGGCGCTTTAACCATTC	TGAACCGAATTTGATCCACG	50.0	45.0	58.0	56.4	429-510 bp	1.0	73.5