Hotspot

clpP-psbB

LSC · 77312-78306 · 995 bp

Back to Zantedeschia

Pi 0

MarkerSeek score 59.10

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 995
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	TCATCTTCCTTGCCATCCTT	GTATAGAGCCATTGAGCCGG	45.0	55.0	56.5	57.9	1182-1186 bp	1.0	67.5
clpP-psbB_p2	TTCATCTTCCTTGCCATCCT	GTATAGAGCCATTGAGCCGG	45.0	55.0	56.5	57.9	1183-1187 bp	1.0	67.5
clpP-psbB_p3	CCAAGTTTCATCTTCCTTGCC	GTATAGAGCCATTGAGCCGG	47.6	55.0	57.4	57.9	1189-1193 bp	1.0	66.9
clpP-psbB_p4	CCCAAGTTTCATCTTCCTTGC	GTATAGAGCCATTGAGCCGG	47.6	55.0	57.4	57.9	1190-1194 bp	1.0	66.9
clpP-psbB_p5	TCATCTTCCTTGCCATCCTT	CGATACCAAGGCAAACCCAT	45.0	50.0	56.5	58.2	1082-1086 bp	1.0	66.3