Hotspot

petA-psbJ

LSC · 69662-70654 · 993 bp

Back to Zantedeschia

Pi 0

MarkerSeek score 58.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 993
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGCTACGCATTCAAGGTCTT	AACCGGTATTCCTGTGATCG	50.0	50.0	58.6	57.4	1184-1185 bp	1.0	70.2
petA-psbJ_p2	CGCTACGCATTCAAGGTCTT	CGGTATTCCTGTGATCGGTT	50.0	50.0	58.6	57.4	1181-1182 bp	1.0	70.2
petA-psbJ_p3	CGCTACGCATTCAAGGTCTT	TCCGGATTAGGCTCATCTCT	50.0	50.0	58.6	57.3	1132-1133 bp	1.0	69.5
petA-psbJ_p4	CGCTACGCATTCAAGGTCTT	CCGGATTAGGCTCATCTCTG	50.0	55.0	58.6	57.3	1131-1132 bp	1.0	69.0
petA-psbJ_p5	ATCCGCTACGCATTCAAGG	CGGTATTCCTGTGATCGGTT	52.6	50.0	58.0	57.4	1184-1185 bp	1.0	67.2