Hotspot

trnM-CAU-trnV-UAC

LSC · 57108-57577 · 470 bp

Back to Zantedeschia

Pi 0.00426

MarkerSeek score 58.50

Primer available yes

Label trnM-CAU-trnV-UAC

Feature Metadata

label_name: trnM-CAU-trnV-UAC
feature_type: igs
region: LSC
length_bp: 470
variable_sites: 2
pi: 0.00426
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-trnV-UAC_p1	GAACCAATGACTATCGCCGT	CTCCCTTGAAAAGCATTGGC	50.0	50.0	58.1	57.6	588-756 bp	1.0	76.7
trnM-CAU-trnV-UAC_p2	TGAACCAATGACTATCGCCG	CTCCCTTGAAAAGCATTGGC	50.0	50.0	58.1	57.6	589-757 bp	1.0	76.7
trnM-CAU-trnV-UAC_p3	GAACCAATGACTATCGCCGT	CGTGTAAACGAGGTGCTCTA	50.0	50.0	58.1	57.4	565-733 bp	1.0	74.7
trnM-CAU-trnV-UAC_p4	TGAACCAATGACTATCGCCG	CGTGTAAACGAGGTGCTCTA	50.0	50.0	58.1	57.4	566-734 bp	1.0	74.7
trnM-CAU-trnV-UAC_p5	CCCATCGATTCCGGGATCTA	CTCCCTTGAAAAGCATTGGC	55.0	50.0	58.4	57.6	638-806 bp	1.0	73.7