Hotspot

psbZ-trnG-GCC

LSC · 38274-38611 · 338 bp

Back to Zantedeschia

Pi 0

MarkerSeek score 34.30

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 338
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TCCCGATGGTTGGTCAAGTA	CGCATCTTTTCCTTGGCAAG	50.0	50.0	58.4	58.0	490 bp	1.0	77.1
psbZ-trnG-GCC_p2	TCCCGATGGTTGGTCAAGTA	CATTACTTCACCATGGGCGA	50.0	50.0	58.4	58.0	527 bp	1.0	76.9
psbZ-trnG-GCC_p3	TTTGCTTCTCCCGATGGTTG	CGCATCTTTTCCTTGGCAAG	50.0	50.0	58.5	58.0	498 bp	1.0	76.2
psbZ-trnG-GCC_p4	CCCGATGGTTGGTCAAGTAA	CGCATCTTTTCCTTGGCAAG	50.0	50.0	57.5	58.0	489 bp	1.0	76.0
psbZ-trnG-GCC_p5	TTTGCTTCTCCCGATGGTTG	CATTACTTCACCATGGGCGA	50.0	50.0	58.5	58.0	535 bp	1.0	76.0