Hotspot

psbA-trnK-UUU

LSC · 1135-1394 · 260 bp

Back to Zantedeschia

Pi 0

MarkerSeek score 54.00

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 260
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	TAGGCTTGTACTTTCGCGTC	CATAGGGAAAGTCGTGTGCA	50.0	50.0	58.0	57.9	414-418 bp	1.0	79.9
psbA-trnK-UUU_p2	TAGGCTTGTACTTTCGCGTC	ACATAGGGAAAGTCGTGTGC	50.0	50.0	58.0	57.9	415-419 bp	1.0	79.9
psbA-trnK-UUU_p3	TAGGCTTGTACTTTCGCGTC	TCGTGTGCAATGAAAACTGC	50.0	45.0	58.0	57.9	403-407 bp	1.0	79.7
psbA-trnK-UUU_p4	TAGGCTTGTACTTTCGCGTC	AATGAAAACTGCAAGCACGG	50.0	45.0	58.0	57.9	395-399 bp	1.0	79.6
psbA-trnK-UUU_p5	TAGGCTTGTACTTTCGCGTC	CGTGTGCAATGAAAACTGCA	50.0	45.0	58.0	58.2	402-406 bp	1.0	79.4