Hotspot

ndhH

Genome · 124813-125994 · 1182 bp

Pi 0.463

MarkerSeek score 79.30

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: Genome
length_bp: 1182
variable_sites: 436
pi: 0.463
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	TCATTTGGAACTGCACCGAA	TTGTGCATGCCTTCACTCTT	45.0	45.0	58.0	58.0	2206 bp	0.5	59.7
ndhH_p2	TCATTTGGAACTGCACCGAA	TAACTGATGGATTGGGCAGC	45.0	50.0	58.0	57.9	1974 bp	0.5	59.3
ndhH_p3	TCATTTGGAACTGCACCGAA	TAAAACGATGTGGCGACCAA	45.0	45.0	58.0	58.1	1834 bp	0.5	58.8
ndhH_p4	TCATTTGGAACTGCACCGAA	TGGCGACCAAAGGGAATTAG	45.0	50.0	58.0	57.9	1824 bp	0.5	58.8
ndhH_p5	TCATTTGGAACTGCACCGAA	GCTGGTCCAAGGCTGAATAA	45.0	50.0	58.0	57.9	1770 bp	0.5	58.8