Hotspot

ndhF

Genome · 136880-139087 · 2208 bp

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Pi 0.469
MarkerSeek score 74.10
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
Genome
length_bp
2208
variable_sites
687
pi
0.469
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.66
markerseek_score
74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 CCTCCTGGTTAACGCCTTTT TACCTGTCTTTCCGAACCCT 50.0 50.0 58.1 58.0 2721 bp 0.5 59.1
ndhF_p2 TCGGAAACCAACCAGGAATG TACCTGTCTTTCCGAACCCT 50.0 50.0 58.1 58.0 2673 bp 0.5 59.1
ndhF_p3 CCTCCTGGTTAACGCCTTTT CATGATCTTCTCCACTGCCC 50.0 55.0 58.1 58.0 2916 bp 0.5 59.0
ndhF_p4 CCTCCTGGTTAACGCCTTTT CCATGATCTTCTCCACTGCC 50.0 55.0 58.1 58.0 2917 bp 0.5 59.0
ndhF_p5 TCGGAAACCAACCAGGAATG CATGATCTTCTCCACTGCCC 50.0 55.0 58.1 58.0 2868 bp 0.5 59.0