Hotspot

ndhE

Genome · 129815-130117 · 303 bp

Pi 0.49

MarkerSeek score 70.30

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: Genome
length_bp: 303
variable_sites: 103
pi: 0.49
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.69
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	GTCGGAGATGGCATCACTTT	CATGCCACAAATAAACGCGT	50.0	45.0	58.0	58.0	1399 bp	0.5	59.6
ndhE_p2	GTCGGAGATGGCATCACTTT	ACGCGTACACTCAAGAAGTC	50.0	50.0	58.0	58.0	1385 bp	0.5	59.4
ndhE_p3	GTCGGAGATGGCATCACTTT	GCTCAACTGCGTTAGTCAGA	50.0	50.0	58.0	57.9	952 bp	0.5	59.2
ndhE_p4	GGGGCTCATTTGTCAACTGA	CATGCCACAAATAAACGCGT	50.0	45.0	58.1	58.0	1249 bp	0.5	59.1
ndhE_p5	TTCATTGGGACCGGTTCTTG	CATGCCACAAATAAACGCGT	50.0	45.0	58.1	58.0	1571 bp	0.5	59.0