Hotspot

ndhD

Genome · 130971-132473 · 1503 bp

Pi 0.475

MarkerSeek score 80.70

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: Genome
length_bp: 1503
variable_sites: 605
pi: 0.475
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	ACGCGTTTATTTGTGGCATG	TAAGGAGACCTGGGCATTGA	45.0	50.0	58.0	58.0	2193 bp	0.5	59.5
ndhD_p2	GACTTCTTGAGTGTACGCGT	TAAGGAGACCTGGGCATTGA	50.0	50.0	58.0	58.0	2207 bp	0.5	59.3
ndhD_p3	CTCAACCCGAGATCTTGAGC	TAAGGAGACCTGGGCATTGA	55.0	50.0	58.1	58.0	2060 bp	0.5	59.2
ndhD_p4	GCTCAACCCGAGATCTTGAG	TAAGGAGACCTGGGCATTGA	55.0	50.0	58.1	58.0	2061 bp	0.5	59.2
ndhD_p5	ACGCGTTTATTTGTGGCATG	CATGGGGATCTCATTGGAGC	45.0	55.0	58.0	58.1	2221 bp	0.5	59.1