Hotspot

chlL

Genome · 116332-117204 · 873 bp

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Pi 0.509
MarkerSeek score 82.80
Primer available yes
Label chlL

Feature Metadata

label_name
chlL
feature_type
gene
region
Genome
length_bp
873
variable_sites
410
pi
0.509
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
chlL_p1 CGGTCCTTTCATCTCTCTGC CCAACCTGATACCTTGACGG 55.0 55.0 58.1 58.0 2290 bp 0.5 59.3
chlL_p2 GGATCATGGGCTGGTTGAAT CCAACCTGATACCTTGACGG 50.0 55.0 57.9 58.0 2243 bp 0.5 59.2
chlL_p3 CGGTCCTTTCATCTCTCTGC CGAGGCTCTGCAAAGATCAT 55.0 50.0 58.1 58.0 1809 bp 0.5 59.1
chlL_p4 CGGTCCTTTCATCTCTCTGC TTCAGAGATCTCCACTGGCA 55.0 50.0 58.1 58.1 1639 bp 0.5 59.0
chlL_p5 GGATCATGGGCTGGTTGAAT CGAGGCTCTGCAAAGATCAT 50.0 50.0 57.9 58.0 1762 bp 0.5 59.0