Hotspot

ccsA

Genome · 132787-133752 · 966 bp

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Pi 0.489
MarkerSeek score 79.90
Primer available yes
Label ccsA

Feature Metadata

label_name
ccsA
feature_type
gene
region
Genome
length_bp
966
variable_sites
385
pi
0.489
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.81
markerseek_score
79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA_p1 AGTGGTTTCGTAGCGGAATC TGAGCACATTGACGATCCAG 50.0 50.0 58.0 58.0 1904 bp 0.5 59.8
ccsA_p2 GAGTGGTTTCGTAGCGGAAT TGAGCACATTGACGATCCAG 50.0 50.0 58.0 58.0 1905 bp 0.5 59.8
ccsA_p3 GTGGTTTCGTAGCGGAATCT TGAGCACATTGACGATCCAG 50.0 50.0 58.0 58.0 1903 bp 0.5 59.8
ccsA_p4 AGTGGTTTCGTAGCGGAATC GAGTGGCGGCATTCTTAGAA 50.0 50.0 58.0 58.0 1786 bp 0.5 59.8
ccsA_p5 GTGGTTTCGTAGCGGAATCT GAGTGGCGGCATTCTTAGAA 50.0 50.0 58.0 58.0 1785 bp 0.5 59.8