Hotspot

rpl32-ndhF

Genome · 135707-136879 · 1173 bp

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Pi 0.463
MarkerSeek score 79.90
Primer available yes
Label rpl32-ndhF

Feature Metadata

label_name
rpl32-ndhF
feature_type
igs
region
Genome
length_bp
1173
variable_sites
445
pi
0.463
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.82
markerseek_score
79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-ndhF_p1 GGGCTCATCATTTGTTGTGC TGAAGTGGAAATTGTGCGGA 50.0 45.0 58.0 58.0 2075 bp 0.5 59.8
rpl32-ndhF_p2 CAAGCATAGAACGAACCGGA TGAAGTGGAAATTGTGCGGA 50.0 45.0 58.0 58.0 2195 bp 0.5 59.8
rpl32-ndhF_p3 TCAAGCATAGAACGAACCGG TGAAGTGGAAATTGTGCGGA 50.0 45.0 58.0 58.0 2196 bp 0.5 59.8
rpl32-ndhF_p4 GGGCTCATCATTTGTTGTGC ACCCAGCTCTGAGGAGAAAT 50.0 50.0 58.0 58.0 1593 bp 0.5 59.7
rpl32-ndhF_p5 CAAGCATAGAACGAACCGGA ACCCAGCTCTGAGGAGAAAT 50.0 50.0 58.0 58.0 1713 bp 0.5 59.7