Hotspot

trnP-GGG-rpl32

Genome · 134209-135496 · 1288 bp

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Pi 0.494
MarkerSeek score 80.10
Primer available yes
Label trnP-GGG-rpl32

Feature Metadata

label_name
trnP-GGG-rpl32
feature_type
igs
region
Genome
length_bp
1288
variable_sites
525
pi
0.494
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.83
markerseek_score
80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-GGG-rpl32_p1 CTGGATCGTCAATGTGCTCA GCACAACAAATGATGAGCCC 50.0 50.0 58.0 58.0 1537 bp 0.5 59.9
trnP-GGG-rpl32_p2 TTCTAAGAATGCCGCCACTC GCACAACAAATGATGAGCCC 50.0 50.0 58.0 58.0 1655 bp 0.5 59.8
trnP-GGG-rpl32_p3 CTGGATCGTCAATGTGCTCA CTGAGTCTATCTCGACCGGT 50.0 55.0 58.0 58.0 1576 bp 0.5 59.5
trnP-GGG-rpl32_p4 CTGGATCGTCAATGTGCTCA TGAGTCTATCTCGACCGGTC 50.0 55.0 58.0 58.0 1575 bp 0.5 59.5
trnP-GGG-rpl32_p5 TTCTAAGAATGCCGCCACTC CTGAGTCTATCTCGACCGGT 50.0 55.0 58.0 58.0 1694 bp 0.5 59.5