Hotspot

ndhE-psaC

Genome · 130118-130586 · 469 bp

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Pi 0.487
MarkerSeek score 76.90
Primer available yes
Label ndhE-psaC

Feature Metadata

label_name
ndhE-psaC
feature_type
igs
region
Genome
length_bp
469
variable_sites
185
pi
0.487
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.81
markerseek_score
76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhE-psaC_p1 GTCGGAGATGGCATCACTTT CATGCCACAAATAAACGCGT 50.0 45.0 58.0 58.0 1399 bp 0.5 59.6
ndhE-psaC_p2 GTCGGAGATGGCATCACTTT TGGGATTAATAAACCCGCGG 50.0 50.0 58.0 58.0 1635 bp 0.5 59.5
ndhE-psaC_p3 GTCGGAGATGGCATCACTTT ACGCGTACACTCAAGAAGTC 50.0 50.0 58.0 58.0 1385 bp 0.5 59.4
ndhE-psaC_p4 GTCGGAGATGGCATCACTTT CTCAAGATCTCGGGTTGAGC 50.0 55.0 58.0 58.1 1531 bp 0.5 59.2
ndhE-psaC_p5 GTCGGAGATGGCATCACTTT GCTCAAGATCTCGGGTTGAG 50.0 55.0 58.0 58.1 1532 bp 0.5 59.2