Hotspot

psbF-psbE

Genome · 68756-68764 · 9 bp

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Pi 0.111
MarkerSeek score 87.50
Primer available yes
Label psbF-psbE

Feature Metadata

label_name
psbF-psbE
feature_type
igs
region
Genome
length_bp
9
variable_sites
1
pi
0.111
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
87.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbF-psbE_p1 GAACGGCCAACCATCTAACT AGAGTCGACAAGAGGTTCCA 50.0 50.0 57.9 58.0 141 bp 1.0 79.7
psbF-psbE_p2 GTACAGCTAGTCCGTGAACG AGAGTCGACAAGAGGTTCCA 55.0 50.0 58.1 58.0 156 bp 1.0 79.6
psbF-psbE_p3 AACGGCCAACCATCTAACTG AGAGTCGACAAGAGGTTCCA 50.0 50.0 58.2 58.0 140 bp 1.0 79.1
psbF-psbE_p4 GTGAACGGCCAACCATCTAA AGAGTCGACAAGAGGTTCCA 50.0 50.0 58.2 58.0 143 bp 1.0 79.1
psbF-psbE_p5 TGAACGGCCAACCATCTAAC AGAGTCGACAAGAGGTTCCA 50.0 50.0 58.2 58.0 142 bp 1.0 79.1