Hotspot

ycf2-trnI-CAU

IRa · 156720-156941 · 222 bp

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Pi 0.0529

MarkerSeek score 82.10

Primer available yes

Label ycf2-trnI-CAU

Feature Metadata

label_name: ycf2-trnI-CAU
feature_type: igs
region: IRa
length_bp: 222
variable_sites: 22
pi: 0.0529
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnI-CAU_p1	TGAATTGAATTGGGTCCATGA	GTTGGGCGCTTTAACCATTC	38.1	50.0	55.4	58.0	357-361 bp	1.0	60.3
ycf2-trnI-CAU_p2	TGAATTGAATTGGGTCCATGA	ATTGGCATGCATCCAGTAGG	38.1	50.0	55.4	58.0	406-410 bp	1.0	59.7
ycf2-trnI-CAU_p3	TGAATTGGGTCCATGAATCT	GTTGGGCGCTTTAACCATTC	40.0	50.0	54.3	58.0	352-356 bp	1.0	59.0
ycf2-trnI-CAU_p4	TGAATTGGGTCCATGAATCT	ATTGGCATGCATCCAGTAGG	40.0	50.0	54.3	58.0	401-405 bp	1.0	58.4
ycf2-trnI-CAU_p5	TGAATTGAATTGGGTCCATGAA	ATTGGCATGCATCCAGTAGG	36.4	50.0	56.2	58.0	406-410 bp	1.0	57.9