Hotspot

trnL-CAA-ycf2

IRa · 148790-149490 · 701 bp

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Pi 0.0666

MarkerSeek score 89.40

Primer available yes

Label trnL-CAA-ycf2

Feature Metadata

label_name: trnL-CAA-ycf2
feature_type: igs
region: IRa
length_bp: 701
variable_sites: 91
pi: 0.0666
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 89.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ycf2_p1	AAATGGTAGACACGCGAGAC	CCCATTCCTTAGCCGGAAAG	50.0	55.0	58.0	58.3	792-808 bp	1.0	89.4
trnL-CAA-ycf2_p2	TGAAATGGTAGACACGCGAG	CCCATTCCTTAGCCGGAAAG	50.0	55.0	58.0	58.3	794-810 bp	1.0	89.4
trnL-CAA-ycf2_p3	AAATGGTAGACACGCGAGAC	GCCGGAAAGATATGTGACCA	50.0	50.0	58.0	57.7	660-797 bp	1.0	89.3
trnL-CAA-ycf2_p4	TGAAATGGTAGACACGCGAG	GCCGGAAAGATATGTGACCA	50.0	50.0	58.0	57.7	662-799 bp	1.0	89.3
trnL-CAA-ycf2_p5	AAATGGTAGACACGCGAGAC	ATTTCCCATTCCTTAGCCGG	50.0	50.0	58.0	57.6	796-812 bp	1.0	89.0