Hotspot

rrn16-trnV-GAC

IRa · 142085-142342 · 258 bp

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Pi 0.0416

MarkerSeek score 88.00

Primer available yes

Label rrn16-trnV-GAC

Feature Metadata

label_name: rrn16-trnV-GAC
feature_type: igs
region: IRa
length_bp: 258
variable_sites: 24
pi: 0.0416
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 88.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-trnV-GAC_p1	CCGACTTGCATGTGTTAAGC	GGCAGGGAGGGGATATAACT	50.0	55.0	57.8	58.0	394-414 bp	1.0	86.4
rrn16-trnV-GAC_p2	CCGACTTGCATGTGTTAAGC	CGTGGTGGAAGTCATCAGTT	50.0	50.0	57.8	57.8	352-372 bp	1.0	86.0
rrn16-trnV-GAC_p3	CCGACTTGCATGTGTTAAGC	TTGACGTGGTGGAAGTCATC	50.0	50.0	57.8	57.8	356-376 bp	1.0	85.9
rrn16-trnV-GAC_p4	GCAGGTTCTTACGCGTTACT	GGCAGGGAGGGGATATAACT	50.0	55.0	58.3	58.0	447-467 bp	1.0	85.5
rrn16-trnV-GAC_p5	TTCTTACGCGTTACTCACCC	GGCAGGGAGGGGATATAACT	50.0	55.0	57.6	58.0	442-462 bp	1.0	85.1