Hotspot

trnN-GUU-trnR-ACG

IRa · 133751-134498 · 748 bp

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Pi 0.141
MarkerSeek score 82.80
Primer available yes
Label trnN-GUU-trnR-ACG

Feature Metadata

label_name
trnN-GUU-trnR-ACG
feature_type
igs
region
IRa
length_bp
748
variable_sites
218
pi
0.141
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-trnR-ACG_p1 CACTACGAAGAAGTTCCGGG CCTTGTTCCTACATGACCCG 55.0 55.0 58.0 58.0 1578-1652 bp 1.0 91.7
trnN-GUU-trnR-ACG_p2 CCACTACGAAGAAGTTCCGG CCTTGTTCCTACATGACCCG 55.0 55.0 58.0 58.0 1579-1653 bp 1.0 91.7
trnN-GUU-trnR-ACG_p3 CACTACGAAGAAGTTCCGGG AAAAGCTCTGTCCTCCCTCT 55.0 50.0 58.0 58.0 1493-1567 bp 1.0 91.6
trnN-GUU-trnR-ACG_p4 CCACTACGAAGAAGTTCCGG AAAAGCTCTGTCCTCCCTCT 55.0 50.0 58.0 58.0 1494-1568 bp 1.0 91.6
trnN-GUU-trnR-ACG_p5 CACTACGAAGAAGTTCCGGG CCTCGCAATCACTAGCCAAT 55.0 50.0 58.0 58.0 1411-1485 bp 1.0 91.6