Hotspot

rps12-clpP

LSC · 75282-75439 · 158 bp

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Pi 0.0913

MarkerSeek score 78.10

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 158
variable_sites: 29
pi: 0.0913
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	CTGAACGAGTCGCACATACA	ATGCAAGCTGTGAAACCAGA	50.0	45.0	58.0	58.0	671-679 bp	1.0	88.8
rps12-clpP_p2	CTGAACGAGTCGCACATACA	TGCAAGCTGTGAAACCAGAT	50.0	45.0	58.0	58.0	670-678 bp	1.0	88.8
rps12-clpP_p3	CTGAACGAGTCGCACATACA	TATAAACTCTCCCGGCGGAT	50.0	50.0	58.0	58.0	723-731 bp	1.0	88.2
rps12-clpP_p4	TACACCGGAGCCTTTCCTTA	TGCAAGCTGTGAAACCAGAT	50.0	45.0	58.1	58.0	1146-1160 bp	0.333333	54.8
rps12-clpP_p5	TACACCGGAGCCTTTCCTTA	TATAAACTCTCCCGGCGGAT	50.0	50.0	58.1	58.0	1199-1213 bp	0.333333	54.7