Hotspot

psbZ-trnG-UCC

LSC · 38428-38750 · 323 bp

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Pi 0.0382
MarkerSeek score 80.10
Primer available yes
Label psbZ-trnG-UCC

Feature Metadata

label_name
psbZ-trnG-UCC
feature_type
igs
region
LSC
length_bp
323
variable_sites
29
pi
0.0382
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-UCC_p1 TTGCTTCTCCTGATGGTTGG CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.8 57.8 478-483 bp 1.0 82.8
psbZ-trnG-UCC_p2 GCTTCTCCTGATGGTTGGTC CGTCTTCTCCTTGGCAAAGA 55.0 50.0 58.3 57.8 476-481 bp 1.0 82.4
psbZ-trnG-UCC_p3 TTGCTTCTCCTGATGGTTGG TACCATTCGACCATATCCGC 50.0 50.0 57.8 57.3 451-456 bp 1.0 78.6
psbZ-trnG-UCC_p4 GCTTCTCCTGATGGTTGGTC TACCATTCGACCATATCCGC 55.0 50.0 58.3 57.3 449-454 bp 1.0 78.3
psbZ-trnG-UCC_p5 CTCCTGATGGTTGGTCAAGT CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.1 57.8 472-477 bp 1.0 77.6