Hotspot

trnH-GUG-psbA

LSC · 312-661 · 350 bp

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Pi 0.0829

MarkerSeek score 82.60

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 350
variable_sites: 65
pi: 0.0829
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	ACAATCTCAGCAAGTAGGCG	GAGCTTAGTTTCCGTCTGGG	50.0	55.0	58.0	58.0	1240 bp	0.166667	46.4
trnH-GUG-psbA_p2	ACAATCTCAGCAAGTAGGCG	GAGTGGGAGCTTAGTTTCCG	50.0	55.0	58.0	58.0	1246 bp	0.166667	46.4
trnH-GUG-psbA_p3	ACAATCTCAGCAAGTAGGCG	GGAGCTTAGTTTCCGTCTGG	50.0	55.0	58.0	58.0	1241 bp	0.166667	46.4
trnH-GUG-psbA_p4	ACAATCTCAGCAAGTAGGCG	GGGAGCTTAGTTTCCGTCTG	50.0	55.0	58.0	58.0	1242 bp	0.166667	46.4
trnH-GUG-psbA_p5	ACAATCTCAGCAAGTAGGCG	TCAGTGCTATGCATGGTTCC	50.0	50.0	58.0	58.0	1002 bp	0.166667	46.2