Hotspot

rps15

SSC · 118096-118368 · 273 bp

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Pi 0

MarkerSeek score 19.60

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 273
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 19.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	AACACCATGCATTGATGGGT	AAGTTACGGAAGGAGGTCCA	45.0	50.0	58.1	58.0	1066 bp	1.0	79.7
rps15_p2	AACACCATGCATTGATGGGT	ATCCCGTGAAATTCTCGAGC	45.0	50.0	58.1	58.1	808 bp	1.0	79.6
rps15_p3	AACACCATGCATTGATGGGT	CTCCTAGACGCCGTAGAGAA	45.0	55.0	58.1	58.0	1129 bp	1.0	79.5
rps15_p4	AACACCATGCATTGATGGGT	GACGCGGAATGGTAGTGAAT	45.0	50.0	58.1	58.1	1199 bp	1.0	79.4
rps15_p5	TAGCTGGCTCTTTGGGGTAT	AAGTTACGGAAGGAGGTCCA	50.0	50.0	58.1	58.0	1290 bp	1.0	79.3