Hotspot

rpoC1

LSC · 24887-26935 · 2049 bp

Back to Yushania

Pi 0.000773

MarkerSeek score 41.50

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2049
variable_sites: 3
pi: 0.000773
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	AGGGAGGAAGTTTGATCGGA	GCGCGGCAAAAATGATGATA	50.0	45.0	58.0	57.9	2173 bp	1.0	78.8
rpoC1_p2	AGGGAGGAAGTTTGATCGGA	ACTAGCGAAATCGGAATGCC	50.0	50.0	58.0	58.4	2154 bp	1.0	76.4
rpoC1_p3	AGGGAGGAAGTTTGATCGGA	GGCATCGCCGAATCTAACTT	50.0	50.0	58.0	58.4	2120 bp	1.0	76.4
rpoC1_p4	AGGGAGGAAGTTTGATCGGA	ATCTCAATCTGGATTCGCGC	50.0	50.0	58.0	58.5	2189 bp	1.0	75.8
rpoC1_p5	GGGAGGAAGTTTGATCGGAA	GCGCGGCAAAAATGATGATA	50.0	45.0	57.2	57.9	2172 bp	1.0	72.9