Hotspot

matK-trnK-UUU

LSC · 3232-3881 · 650 bp

Back to Yushania

Pi 0.00171

MarkerSeek score 48.00

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 650
variable_sites: 5
pi: 0.00171
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	ACTCCTGAAAGAGAAGTGGGT	CGTGCGGTGTAATTCCATTG	47.6	50.0	58.3	57.8	822-865 bp	1.0	68.6
matK-trnK-UUU_p2	ACTCCTGAAAGAGAAGTGGG	CGTGCGGTGTAATTCCATTG	50.0	50.0	56.5	57.8	822-865 bp	1.0	66.8
matK-trnK-UUU_p3	CTCCTGAAAGAGAAGTGGGT	CGTGCGGTGTAATTCCATTG	50.0	50.0	56.5	57.8	821-864 bp	1.0	66.8
matK-trnK-UUU_p4	ACTCCTGAAAGAGAAGTGGGT	TGGGTTGCTAACTCAATGGT	47.6	45.0	58.3	57.0	763-806 bp	1.0	62.3
matK-trnK-UUU_p5	ACTCCTGAAAGAGAAGTGGGTA	CGTGCGGTGTAATTCCATTG	45.5	50.0	58.1	57.8	822-865 bp	1.0	61.9