Hotspot

ndhA

SSC · 122696-124895 · 2200 bp

Pi 0.00513

MarkerSeek score 52.80

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2200
variable_sites: 33
pi: 0.00513
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CCGGTCACCATAGGGAACAT	TGGGAGAAGTTGATCGTTGA	55.0	45.0	59.2	56.5	2332-2345 bp	1.0	60.5
ndhA_p2	CCGGTCACCATAGGGAACAT	TGGGAGAAGTTGATCGTTGAA	55.0	42.9	59.2	57.2	2332-2345 bp	1.0	58.5
ndhA_p3	CGGTCACCATAGGGAACATA	TGGGAGAAGTTGATCGTTGA	50.0	45.0	56.4	56.5	2331-2344 bp	1.0	57.2
ndhA_p4	CCGGTCACCATAGGGAACAT	ATGGGAGAAGTTGATCGTTGA	55.0	42.9	59.2	57.0	2333-2346 bp	1.0	56.8
ndhA_p5	CCGGTCACCATAGGGAACA	TGGGAGAAGTTGATCGTTGA	57.9	45.0	58.7	56.5	2332-2345 bp	1.0	56.2