Hotspot

trnN-GUU-trnR-ACG

IRa · 132688-133311 · 624 bp

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Pi 0.00382
MarkerSeek score 58.50
Primer available yes
Label trnN-GUU-trnR-ACG

Feature Metadata

label_name
trnN-GUU-trnR-ACG
feature_type
igs
region
IRa
length_bp
624
variable_sites
6
pi
0.00382
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-trnR-ACG_p1 CCGTTGTTCCTCAGTAGCTC CAGAGGATTAGAGCACGTGG 55.0 55.0 58.0 57.8 740-765 bp 1.0 81.5
trnN-GUU-trnR-ACG_p2 CCGTTGTTCCTCAGTAGCTC GAACAGATCCTGTGGAGACG 55.0 55.0 58.0 57.7 777-802 bp 1.0 80.8
trnN-GUU-trnR-ACG_p3 TGGTCGTAGGTTCGAATCCT CAGAGGATTAGAGCACGTGG 50.0 55.0 58.2 57.8 691-716 bp 1.0 80.5
trnN-GUU-trnR-ACG_p4 TGGTCGTAGGTTCGAATCCT GAACAGATCCTGTGGAGACG 50.0 55.0 58.2 57.7 728-753 bp 1.0 79.8
trnN-GUU-trnR-ACG_p5 CCGTTGTTCCTCAGTAGCTC GGGAACAGATCCTGTGGAGA 55.0 55.0 58.0 58.4 779-804 bp 1.0 79.7