Hotspot

ndhF-rpl32

SSC · 115247-116209 · 963 bp

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Pi 0.00356
MarkerSeek score 54.70
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
963
variable_sites
11
pi
0.00356
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGCGGGAATAAGCAGAAGTC TGCAGCTAAATTTCCCTTTTTCT 50.0 34.8 58.0 57.2 1135-1143 bp 1.0 50.7
ndhF-rpl32_p2 TATTTTTGGTTGCTGCGGGA TGCAGCTAAATTTCCCTTTTTCT 45.0 34.8 58.4 57.2 1148-1156 bp 1.0 47.8
ndhF-rpl32_p3 TTTGGTTGCTGCGGGAATAA TGCAGCTAAATTTCCCTTTTTCT 45.0 34.8 58.4 57.2 1144-1152 bp 1.0 47.8
ndhF-rpl32_p4 TTTTGGTTGCTGCGGGAATA TGCAGCTAAATTTCCCTTTTTCT 45.0 34.8 58.4 57.2 1145-1153 bp 1.0 47.8
ndhF-rpl32_p5 TGCGGGAATAAGCAGAAGTC TGCAGCTAAATTTCCCTTTTTCTT 50.0 33.3 58.0 57.8 1135-1143 bp 1.0 47.8