Hotspot

trnR-ACG-trnN-GUU

IRb · 111003-111626 · 624 bp

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Pi 0.00382
MarkerSeek score 58.50
Primer available yes
Label trnR-ACG-trnN-GUU

Feature Metadata

label_name
trnR-ACG-trnN-GUU
feature_type
igs
region
IRb
length_bp
624
variable_sites
6
pi
0.00382
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-trnN-GUU_p1 CAGAGGATTAGAGCACGTGG CCGTTGTTCCTCAGTAGCTC 55.0 55.0 57.8 58.0 740-765 bp 1.0 81.5
trnR-ACG-trnN-GUU_p2 GAACAGATCCTGTGGAGACG CCGTTGTTCCTCAGTAGCTC 55.0 55.0 57.7 58.0 777-802 bp 1.0 80.8
trnR-ACG-trnN-GUU_p3 CAGAGGATTAGAGCACGTGG TGGTCGTAGGTTCGAATCCT 55.0 50.0 57.8 58.2 691-716 bp 1.0 80.5
trnR-ACG-trnN-GUU_p4 GAACAGATCCTGTGGAGACG TGGTCGTAGGTTCGAATCCT 55.0 50.0 57.7 58.2 728-753 bp 1.0 79.8
trnR-ACG-trnN-GUU_p5 GGGAACAGATCCTGTGGAGA CCGTTGTTCCTCAGTAGCTC 55.0 55.0 58.4 58.0 779-804 bp 1.0 79.7