Hotspot

trnT-GGU-psbD

LSC · 32952-34046 · 1095 bp

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Pi 0.00488
MarkerSeek score 53.30
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1095
variable_sites
12
pi
0.00488
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG CGGACCAACCTACAAAAACG 55.0 50.0 58.1 57.3 1250-1258 bp 1.0 75.4
trnT-GGU-psbD_p2 GTGGTAGAGTAACGCCATGG GCCGGACCAACCTACAAAAA 55.0 50.0 58.1 58.7 1252-1260 bp 1.0 75.4
trnT-GGU-psbD_p3 TCAGTGGTAGAGTAACGCCA CGGACCAACCTACAAAAACG 50.0 50.0 58.1 57.3 1253-1261 bp 1.0 75.3
trnT-GGU-psbD_p4 GTGGTAGAGTAACGCCATGG GAAAGAGCAATAGGCCGGAC 55.0 55.0 58.1 58.7 1265-1273 bp 1.0 75.2
trnT-GGU-psbD_p5 TCAGTGGTAGAGTAACGCCA GCCGGACCAACCTACAAAAA 50.0 50.0 58.1 58.7 1255-1263 bp 1.0 75.2