Hotspot

trnE-UUC-trnT-GGU

LSC · 32378-32879 · 502 bp

Back to Yucca

Pi 0.00476
MarkerSeek score 54.20
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
502
variable_sites
7
pi
0.00476
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 CGAATCCCCGTTTTCTCCTT GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 606-614 bp 1.0 81.2
trnE-UUC-trnT-GGU_p2 CGAATCCCCGTTTTCTCCTT CCATGGCGTTACTCTACCAC 50.0 55.0 57.9 58.1 585-593 bp 1.0 80.8
trnE-UUC-trnT-GGU_p3 TTTCGTAGTACCCTACCCCC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 635-643 bp 1.0 80.7
trnE-UUC-trnT-GGU_p4 GGAAGTCGAATCCCCGTTTT GAACCGATGACTTACGCCTT 50.0 50.0 58.2 58.0 612-620 bp 1.0 80.6
trnE-UUC-trnT-GGU_p5 CGAATCCCCGTTTTCTCCTT TGGCGTTACTCTACCACTGA 50.0 50.0 57.9 58.1 582-590 bp 1.0 80.6